中心成員
翁雨蕙

翁雨蕙 Dr. Wong, Yu-Hui

Phone: +886-2-28267000 ext. 6252
E-mail: yuhui.wong@gmail.com

POSITION TITLE/AFFILIATIONS

Assistant Researcher/Brain Research Center

EDUCATION/TRAINING

2003-2009 Ph.D. Neuroscience, National Yang-Ming University, Taipei, Taiwan
2010-2014 Postdoc in Neurobiology, University of California, Berkeley, USA
2014-2016 Postdoc in Neurodegenerative disease, National Yang-Ming University, Taipei, Taiwan

CURRENT RESEARCH

Alzheimer's disease (AD) is the most common form of age-related dementia, characterized by progressive memory loss and cognitive disturbance.  Although the majority of AD cases are sporadic, about 5% of cases are inherited in an autosomal dominant pattern as familial AD (FAD) and manifest at an early age.  Mutations in the amyloid precursor protein (APP), presenilin 1 (PSEN1) or presenilin 2 (PSEN2) gene account for the majority of early-onset FAD.  Now we are generating human induced pluripotent stem cells (hiPSCs) derived from AD patients’ blood or fibroblasts harboring the FAD APP or PSEN1 mutation, and double-variant alleles for APOE ε4.  Moreover, we will use the latest gene-editing tool, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9), to correct gene mutation in AD-iPSCs.  Then, we will differentiate these AD-iPSC (with or without gene correction) lines to the neuronal lineage to study whether these neurons have mature phenotypic and physiological properties, as well as AD-like biochemical features.  In the future, FAD hiPSCs harboring disease properties can be used as humanized models to test novel diagnostic methods and therapies and explore novel hypotheses for AD pathogenesis.

SELECTED PUBLICATIONS in the past 5 years
  1. Chen HR, Juan HC, Wong YH, Tsai JW, Fann MJ. Cdk12 regulates neurogenesis and late-born neuronal migration in the developing cerebral cortex. Cereb Cortex 27(3):2289-2302.


  2. Wong YH, Lee CM, Xie W, Cui B, Poo MM (2015). Activity-dependent BDNF release via endocytic pathways is regulated by complexin and synaptotagmin-6. Proc Natl Acad Sci U S A. 112(32):E4475-4484 13:714-212013


  3. Wang YC, Juan HC, Wong YH, Kuo WC, Lu YL, Lin SF, Lu CJ, Fann MJ (2013). Protogenin prevents premature apoptosis of rostral cephalic neural crest cells by activating the α5β1-integrin. Cell Death Dis. 4:e651