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師資 faculty

實驗神經組

 

蔡金吾 副教授
Ph.D.

陽明大學 腦科學研究所 專任副教授

Tel: (02) 2826-7305
Fax: (02) 2827-3123

E-mail : tsaijw@ym.edu.tw

研究室網頁

 

 

主要學歷

  • 2002 - 2008: 哥倫比亞大學(Columbia University)細胞分子及生物物理學博士(Cellular, Molecular, and Biophysical Studies)(指導教授:Richard Vallee)
  • 1998 - 2000: 國立陽明大學微生物及免疫學碩士(指導教授:林奇宏)
  • 1994 - 1998: 國立台灣大學物理學及動物學雙學士

現職

  • 2018- 國立陽明大學腦科學研究所 專任副教授

經歷

  • 2012-2018 國立陽明大學腦科學研究所 專任助理教授
  • 2010-2012 Genentech Inc. 研究及發展部生醫影像組科學經理 (Scientific Manager)
  • 2009-2010 加州大學舊金山分校 (UCSF) 幹細胞與再生醫學研究中心及神經科博士後研究員
  • 2008-2009 紐約大學 (NYU) 分子神經生物學博士後研究員

重要獎項與榮譽

  • 2018, 2015 國立陽明大學「教學優良獎」
  • 2017, 2014 科技部「優秀年輕學者研究計畫」
  • 2015-2018 連續四年獲國立陽明大學醫學院「醫學系學生網路教學評估優良教師」
  • 2012 國科會「延攬特殊優秀人才」補助計畫
  • 2011 Recognition Award, Genentech Inc.
  • 2011 Ruth L. Kirschstein National Research Service Awards for Individual Postdoctoral Fellows, National Institutes of Health, USA
  • 2010 “Study Abroad” Postdoctoral Fellowship, National Science Council, Taiwan
  • 2008 Spinal Cord Injury Research Program Postdoctoral Fellowship Award, Spinal Cord Injury Research Board (SCIRB), New York State Department of Health
  • 2008 The Rover Award for Outstanding Achievement in Anatomy and Cell Biology
  • 2008 Ph.D. degree with Distinction, Columbia University
  • 2007 International Brain Research Organization Travel Grant for the World Congress of Neuroscience
  • 2007 The British Council Stem Cell Workshop Scholarship
  • 2006 AAAS/Science Program for Excellence in Science
  • 2006 NIH National Graduate Student Research Festival Scholarship
  • 2006 Taiwanese Bioscientist Association Student Travel Award
  • 2005 The Brunie Prize in Neural Stem Cell Research
  • 2005 American Society for Cell Biology (ASCB) Predoctoral Student Travel Award
  • 2000 The Best Student Paper Award, International Society for Optical Engineering
  • 2000 國立陽明大學「優秀碩士論文獎」
  • 1998 吳健雄科學獎,吳健雄教育基金會
  • 1997 行政院國家科學委員會「大專生暑期研究計畫」
  • 1996, 1994 國立台灣大學「成績優良(書卷)獎」
  • 1994 「高級中學自然科學資賦優異學生」保送國立台灣大學物理學系
  • 1994 獲選第25屆「國際物理奧林匹亞競賽 (International Physics Olympiad) 」國家代表隊
  • 1994 獲選參加「亞太數學奧林匹亞競試 (Asian Pacific Mathematics Olympiad) 」
  • 1994 「全國科學才能青年選拔活動高中數學競試」二等獎
  • 1994 當選「台灣省、台北市暨高雄市高級中學特殊優良學生」
  • 1994 「中華民國第34屆中小學科學展覽」物理科高中組第二名
  • 1994 「高級中學數學及自然學科競賽」物理科決賽第二名

研究概況

運用先進的跨領域技術(橫跨細胞分子、光學影像、程式設計、臨床與生物科技)研究中樞神經系統之發育、神經幹細胞複製與分化之動態過程。並延伸到大腦發育神經元突觸之相互連結,以及在神經退化、中風以及腦損傷過程中,腦部細胞之動態變化,期望以這樣的整合系統,發展出以神經再生為標的之幹細胞療法。歡迎有興趣或具有上述任一背景之學生、研究及醫療人員加入我們的行列。

主要技術:光學顯微技術、共軛焦顯微鏡 (Confocal microscopy) 、雙光子雷射掃描顯微鏡 (Two-photon laser scanning microscopy) 、腦組織切片培養、活細胞間歇顯微造影 (in vivo time-lapse microscopy)、神經幹細胞培養 (neural stem cell culture)、子宮內電穿孔 (in utero electroporation) 、數位影像處理(Matlab)。

應邀演講

1. Invited speaker in the 23rd Congress of Chinese Pediatric Society, Xiamen, China (2018)
2. Invited speaker in the 12th NYMU-UCSD Bilateral Symposium, La Jolla, CA (2018)
3. Invited speaker in the Annual Meeting of the Association for the Study of Neurons and Diseases, Taipei, Taiwan (2018)
4. Invited speaker in the Cross-Strait Epilepsy Gene Research Forum, Taipei, Taiwan (2018)
5. Invite speaker in第三屆兩岸四地癲癇病學青年峰會,中國長春 (2018)
6. Keynote speaker in the Asia Pacific Medical Student Symposium (APMSS), Taipei, Taiwan (2017)
7. Invited speaker in the 10th NYMU-UCSD Bilateral Symposium, La Jolla, CA (2016)
8. Invited symposium speaker in the 14th Meeting of the Asian-Pacific Society for Neurochemistry (APSN), Kuala Lumpur, Malaysia (2016)
9. Invited speaker in Focus on Microscopy 2016 conference , Taipei, Taiwan (2016)
10. Invited symposium speaker in the 6th Federation of Asia-Oceanian Neuroscience Society (FAONS) Congress and the 11th Biennial Conference of CNS, Wuzhen, China (2015)
11. Invited speaker in the 25th ISN-APSN-ASN Joint Meeting Satellite Meeting, Cairns, Australia (2015)
12. Invited speaker in the 4th Symposium of World Association of Chinese Epileptologists (WACE), Kaohsiung, Taiwan (2014)
13. Invited speaker in the 4th Annual World Congress of Molecular & Cell Biology Meeting, Dalian, China (2014)
14. Invited speaker in the 12th Annual Meeting of the Taiwan Epilepsy Society, Taipei, Taiwan (2014)
15. Invited speaker in the Conference of the Federation of Asian Societies for Molecular Imaging, Taipei, Taiwan (2013)
16. Invited speaker in the Combined ASBMB, ASPS, and ANZSCDB Annual Meeting (ComBio), Perth, Australia (2013)
17. Invited speaker in the International Symposium on Development, Morphogenesis, and Stem Cells, Taipei, Taiwan (2013)
18. Invited speaker in the 6th NYMU-UCSD Bilateral Symposium, La Jolla, CA (2012)
19. Invited speaker in the Annual Meeting of Taiwan Neuroscience Society, Taipei, Taiwan (2011)
20. Invited speaker in the 4th NYMU-UCSD Bilateral Symposium, La Jolla, CA (2010)
21. Speaker in the 39th Neuroscience Meeting, Chicago, IL, Society for Neuroscience (2009)
22. Invited speaker in the 2nd Global COE Retreat, Hokuto, Japan, University of Tokyo (2009)
23. Invited speaker in the 1st Tohoku Neuroscience GCOE conference “From Genes to Development and Behavior”, Zao, Japan, Tohoku University (2008)
24. Speaker in the “Stem cells, axon guidance and migration in the developing and adult brain” conference, Cairns, Australia, IBRO (2007)
25. Invited speaker in the 46th Cell Biology Meeting, San Diego, CA, ASCB (2006)
26. Invited speaker in the CAS Symposium on Model Organisms and Diseases, Beijing, China, Chinese Academy of Sciences (2006)
27. Speaker in the 36th Neuroscience Meeting, Atlanta, GA, Society for Neuroscience (2006)
28. Speaker in the 35th Neuroscience Meeting, Washington, DC, Society for Neuroscience (2005)
29. Invited speaker in the 44th Cell Biology Meeting, Washington, DC, ASCB (2004)
30. Invited speaker in Photonics Taiwan 2000, Taipei, Taiwan, Int’l Society of Optical Engineering (2000)

指導學生獲獎:

2018 碩士班康以寧、張馨云、劉臻獲the 15th Meeting of the Asian-Pacific Society for Neurochemistry (APSN) Travel Award並獲選Oral presentation
2018 碩士班王竣弘獲「陽明大學學術論文比賽」佳作獎
2018 碩士班康以寧獲「紀念尹珣若先生品學及論文優良獎學金」優等獎
2018 碩士班劉臻獲陽明大學「生技教育基金」獎學金
2017 醫學系洪學宇獲健康科學文教基金會「醫學系學生暑期研究計畫成果發表亞軍
2017 碩士班張馨云獲「罕見疾病基金會獎學金」
2017 博士班黃嘉偉、鄭皓元獲陽明大學「生技教育基金」獎學金
2017 碩士班鄭皓元獲陽明大學「修讀博士班獎學金」
2017 碩士班蕭景如獲「紀念尹珣若先生品學及論文優良獎學金」入選獎
2017 醫學系洪學宇獲健康科學文教基金會「醫學系學生暑期研究計畫」
2017 生科系柳維思、醫學系洪學宇獲科技部「大專生研究計畫」
2016 碩士班陳怡安獲「陽明大學學術論文比賽」優等獎
2016 碩士班陳怡安獲「紀念尹珣若先生品學及論文優良獎學金」入選獎
2016 生科系張馨云、葉思妤獲科技部「大專生研究計畫」
2015 醫學系陳紹倫、廖柔謙獲科技部「大專生研究創作獎」
2015 碩士班張家祥獲「紀念尹珣若先生品學及論文優良獎學金」優等獎
2015 醫學系廖柔謙、林伯禧獲科技部「大專生研究計畫」
2014 博士班粘芳馨獲選Cold Spring Harbor Asia Meeting on Neurobiology口頭報告
2014 醫學系廖柔謙獲健康科學文教基金會「獎助暑期大專生參與研究計畫」
2014 醫學系陳紹倫、廖柔謙獲科技部「大專生研究計畫」
2013 碩士班粘芳馨獲「紀念尹珣若先生品學及論文優良獎學金」入選獎



專利

1. 林奇宏、蔡金吾、易永祥 「細胞側視系統與方法」中華民國發明專利#00594011 (2004-2020).

發表論文

  1. Chang CH, Zanini M, Shirvani H, Cheng JS, Yu H, Feng CH, Mercier AL, Hung SY, Forget A, Wang CH, Cigna SM, Lu IL, Chen WY, Leboucher S, Wang WJ, Ruat M, Spassky N, Tsai JW*, Ayrault O* (2019) Atoh1 requires primary cilia for the expansion of granule neuron progenitors by modulating centriolar satellites. Dev Cell, accepted. (* corresponding)
  2. Hsiao CJ, Chang CH, Ibrahim RB, Lin IH, Wang CH, Wang WJ, and Tsai JW* (2019) Gli2 modulates cell cycle re-entry through autophagy-mediated regulation on the length of primary cilia. J Cell Sci, accepted. (* corresponding)
  3. Chen YA, Lu IL, Tsai JW* (2018) Contactin-1/F3 regulates neuronal migration and morphogenesis through modulating RhoA activity. Front Mol Neurosci, accepted. (* corresponding)
  4. Kilander MBC, Wang CH, Chang CH, Nestor JE, Herold K, Tsai JW, Nestor MW, Lin YC (2019) A rare human CEP290 variant disrupts the molecular integrity of the primary cilium and impairs Sonic Hedgehog machinery. Sci Rep, accepted.
  5. Lu IL, Chen C, Tung CY, Chen HH, Pan JP, Chang CH, Cheng JS, Chen YA, Wang CH, Huang CW, Kang YN, Chang HY, Li LL, Chang KP, Shih YH, Lin CH, Kwan SY, Tsai JW* (2018) Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice. Nat Commun, 9(1):2498. (* corresponding)
  6. Valente P, Romei A, Fadda M, Sterlini B, Lonardoni D, Forte N, Fruscione F, Castroflorio E, Michetti C, Giansante G, Valtorta F, Tsai JW, Zara F, Nieus T, Corradi A, Fassio A, Baldelli P, Benfenati F (2018) Constitutive inactivation of the PRRT2 gene alters short-term synaptic plasticity and promotes network hyperexcitability in hippocampal neurons. Cereb Cortex, in press.
  7. Chen JL, Chang CH, Tsai JW* (2018) Gli2 rescues delays in brain development induced by Kif3a dysfunction. Cereb Cortex, in press. (* corresponding)
  8. Jheng GW, Hur SS, Chang CM, Wu CC, Cheng JS, Lee HH, Chung BC, Wang YK, Lin KH, del ?lamo JC, Chien S, Tsai JW* (2018). Lis1 dysfunction leads to traction force reduction and cytoskeletal disorganization during cell migration. Biochem Biophys Res Commun, 497, 869-75. (* corresponding)
  9. Chakraborty S, Karmenyan A, Tsai JW, Chiou A (2017) Inhibitory effects of curcumin and cyclocurcumin in 1-methyl-4-phenylpyridinium (MPP+) induced neurotoxicity in differentiated PC12 cells. Sci Rep, 7, 16977.
  10. Liu YH, Tsai JW, Chen JL, Yang WS, Chang PC, Cheng PL, Turner DL, Yanagawa Y, Wang TW, Yu JY (2017) Ascl1 promotes tangential migration and confines migratory routes by induction of Ephb2 in the telencephalon. Sci Rep, 7, 42895.
  11. Chen HR, Juan HC, Wong YH, Tsai JW, Fann MJ (2017) Cdk12 regulates neurogenesis and late-arising neuronal migration in the developing cerebral cortex. Cereb Cortex, 27:2289-302.
  12. Tsai MH, Kuo PW, Myers CT, Li SW, Lin WJ, Fu TY, Chang HY, Mefford HC, Chang YC*, Tsai JW* (2016). A novel DCX missense mutation in a family with X-linked lissencephaly and subcortical band heterotopia syndrome inherited from a low-level somatic mosaic mother: Genetic and functional studies. Eur J Paediatr Neurol, 20, 788-94. (* corresponding)
  13. Liu YT, Nian FS, Chou WJ, Tai CY, Kwan SY, Chen C, Kuo PW, Lin PH, Chen CY, Huang CW, Lee YC, Soong BW*, Tsai JW* (2016) PRRT2 mutations lead to neuronal dysfunction and neurodevelopmental defects. Oncotarget, 7, 39184-96. (* corresponding)
  14. Ma L*, Qiao Q*, Tsai JW*, Yang G, Li W, Gan WB (2016) Experience-dependent plasticity of dendritic spines of layer 2/3 pyramidal neurons in the mouse cortex. Dev Neurobiol, 76, 277-286. (*equal contribution)
  15. Qiao Q, Ma L, Li W, Tsai JW, Yang G, Gan WB (2016) Long-term stability of axonal boutons in the mouse barrel cortex. Dev Neurobiol, 76, 252-261.
  16. Chakraborty S, Nian FS, Tsai JW, Karmenyan A, Chiou A (2016) Quantification of the metabolic state in cell-model of Parkinson’s disease by fluorescence lifetime imaging microscopy. Sci Rep, 6, 19145.
  17. Cheng CY, Wu JC, Tsai JW, Nian FS, Wu PC, Kao LS, Fann MJ, Tsai SJ, Liou YJ, Tai CY, Hong CJ (2015) ENU mutagenesis identifies mice modeling Warburg Micro syndrome with sensory axon degeneration caused by a deletion in Rab18. Exp Neurol, 267, 143-151.
  18. Tsai JW & Vallee RB (2011) Live microscopy of neural stem cell migration in brain slices. Methods Mol Biol, 750:131-42.
  19. Wang X, Tsai JW, Lamonica B & Kriegstein AR (2011) A new subtype of progenitor cell in the mouse embryonic neocortex. Nat Neurosci, 14, 555-561. (Cover; featured in News and Views)
  20. Tsai JW*, Lian WN*, Kemal SK, Kriegstein AR & Vallee RB (2010) Kinesin 3 and cytoplasmic dynein mediate interkinetic nuclear migration in neural stem cells. Nat Neurosci, 13, 1463-1471. (*equal contribution)
  21. Wang X, Tsai JW, Imai JH, Lian WN, Vallee RB & Shi SH (2009) Asymmetric inheritance of centrosome maintains neural progenitors during mammalian neocortical neurogenesis. Nature 461, 947-955. (Cover; featured in News and Views)
  22. Vallee RB, Seale GE & Tsai JW (2009) Motor protein mechanisms for neuronal migration and growth cone guidance. Trends Cell Biol 19, 347-355.
  23. Tsai JW, Bremner KH & Vallee RB (2007) Dual subcellular roles for LIS1 and dynein in radial neuronal migration in live brain tissue. Nat Neurosci 10, 970-979.
  24. Vallee RB & Tsai JW (2006) The cellular roles of the lissencephaly gene LIS1, and what they tell us about brain development. Genes Dev 20, 1384-1393.
  25. Hsieh CF, Chang BJ, Pai CH, Chen HY, Tsai JW, Yi YH, Chiang YT, Wang DW, Chi S, Hsu L & Lin CH (2006) Stepped changes of monovalent ligand-binding force during ligand-induced clustering of integrin ?圂Ib??3. J Biol Chem 281, 25466-25474.
  26. Tsai JW, Chen Y, Kriegstein AR & Vallee RB (2005) LIS1 RNAi blocks neural stem cell division, morphogenesis, and motility at multiple stages. J Cell Biol 170, 935-945. (Cover and highlight paper)
  27. Hsieh CF, Chang BJ, Pai CH, Chen HY, Chi S, Hsu L, Tsai JW & Chi-Hung Lin (2004) Identification of stepped changes of binding affinity during interactions between the disintegrin rhodostomin and integrin ?圂Ib??3 in living cells using optical tweezers. Proc SPIE 5514, 215-224.
  28. Liu JC, Hwang WL, Chen MS, Tsai JW & Lin CH (2001) Wavelet-based active contour model for object tracking. ICIP Proc 3, 206-209.
  29. Tsai JW, Liao BY, Huang CC, Hwang WL, Wang DW, Chiou AE & Lin CH (2000) Applications of optical tweezers and an integrated force measurement module for biomedical research. Proc SPIE 4082, 213-221. (Best Student Paper Award)
  30. 林奇宏、蔡金吾 (2000).「雷射光鉗技術在生物作用力研究上的應用」物理雙月刊22卷5期, p500-505.
  31. Lian WN, Tsai JW, Yu PM, Wu TW, Yang SC, Chau YP & Lin CH (1999) Targeting of aminopeptidase N to bile canaliculi correlates with secretory activities of the developing canalicular domain. Hepatology 30, 748-760.

Nat Neurosci 14(5) cover

Nature 461(7266) cover

JCB 170(6) cover

 

會議摘要

1. Tsai JW, Hedehus M, Kallop D, Weimer R. An interactive semi-automatic dendritic spine detection tool for efficient quantification of synaptic dynamics in vivo. Imaging in 2020 VII, Jackson Hole, WY (2011)
2. Wang X, Tsai JW, Lamonica B, Kriegstein AR. The cellular roles for MCPH genes CDK5RAP2 and CPAP in cortical development. ISSCR 8th Annual Meeting, San Francisco, CA (2010)
3. Lian WN, Tsai JW & Vallee RB. Cytoplasmic dynein and nonconventional kinesin responsible for bidirectional interkinetic nuclear oscillations in neuroepithelial cells. The 39th Neuroscience Meeting, Chicago, IL (2009)
4. Tsai JW. From neurogenesis to synaptical genesis: a long journey for cortical neurons. The 2nd Global COE Retreat, Hokuto, Japan (2009)
5. Tsai JW, Lian WN & Vallee RB. The molecular basis for interkinetic nuclear oscillations in the mammalian neuroepithelium. The 48th Cell Biology Meeting, San Francisco, CA (2008)
6. Tsai JW, Bremner KH & Vallee RB. Subcellular roles for LIS1 and dynein in radial migration of neurons during embryonic cortical development. The 1st Tohoku Neuroscience Global-COE Conference, Zao, Japan (2008)
7. Tsai JW, Bremner KH & Vallee RB. Dynein and LIS1 Transport the entire microtubule cytoskeleton through in migrating neural precursors, and myosin IIb brings up the rear. The 47th Cell Biology Meeting, Washington, DC (2007)
8. Tsai JW & Vallee RB. Dual subcellular roles for lis1 and dynein in radial migration of neurons during embryonic cortical development. IBRO World Congress of Neuroscience, Melbourne, Australia (2007)
9. Tsai JW & Vallee RB. Triple labeling of migrating neural progenitor cells in live brain tissue reveals novel roles for cytoplasmic dynein and LIS1 in centrosomal, nuclear, and microtubule behavior. The 46th Cell Biology Meeting, San Diego, CA (2006)
10. Tsai JW & Vallee RB. Direct observation of microtubule dynamics in live brain tissue. The 46th Cell Biology Meeting, San Diego, CA (2006)
11. Tsai JW. Studying pathogenesis of lissencephaly in the rodent model using RNA interference and in utero electroporation. CAS Symposium on Model Organisms and Diseases, Beijing, China (2006)
12. Tsai JW & Vallee RB. Triple labeling of neural progenitor cells in live brain tissue reveals novel roles for cytoplasmic dynein and LIS1 in centrosomal and nuclear behavior. The 36th Neuroscience Meeting, Atlanta, GA (2006)
13. Tsai JW et al. Multiple effects of LIS1 RNAi on neural progenitor cell division, polarization, axonogenesis, and motility. The 45th Cell Biology Meeting, San Francisco, CA (2005).
14. Tan SC, Tsai JW et al. Dynein light intermediate chains: A comparison of distribution and function in fibroblasts and developing neurons. The 45th Cell Biology Meeting, San Francisco, CA (2005).
15. Tsai JW et al. LIS1 RNAi blocks neural progenitor cells at multiple stages in the radial migration pathway. The 35th Neuroscience Meeting, Washington, DC (2005).
16. Vallee RB, Tsai JW. Role of cytoplasmic dynein and LIS1 in neural progenitor cell migration and division. International Dynein Workshop, Kobe, Japan (2005).
17. Vallee RB, Tsai JW. Role of cytoplasmic dynein in brain developmental and neurodegerative disease. Experimental Biology Conference, San Diego, CA (2005).
18. Tsai JW et al. Interference with LIS1 and dynactin arrests neuronal migration at the multipolar stage and interferes with neocortial expansion. Mol. Biol. Cell 15, 173a (2004).



 
 
 

 

 

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國立陽明大學腦科學研究所

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9/17/2015更新 | 版權所有© 2015 陽明大學腦科學研究所 | 網頁設計: 蔡金吾 助理教授