中心成員

朱業修 Dr. Chu, Yeh-Shiu

Phone: +886-2-28267000 ext. 6226, 7302
E-mail: y27632@gmail.com

POSITION TITLE/AFFILIATIONS

Assistant Researcher/Brain Research Center

EDUCATION/TRAINING

2000-2005 Ph.D, in  Molecular and Cell Biology.   University of Paris  VI, France
2006-2007 Postdoc in  Cell Biology/Biophysics. L’Institut Curie, France
2007-2011 Senior Research Fellow in Light  MicroscopyMechanobiology.Institute of Molecular and Cell Biology, Singapore
2004-2008 MS in Physiology/Neuroendocrinology.National Yang-Ming University

CURRENT RESEARCH

My research focuses on investigating the molecular mechanisms of Cadherin-mediated cell adhesion, and the interplay between Cadherin and cortical F-Actin from the perspective of mechanobiology. To gain information on molecular interactions of cell adhesion in depth is fundamentally important for providing insights in understanding basic cellular physiology, such as formation of Adherens Junctions in epithelia and maintenance of synapses in neuron. However, at the intercellular adhesive interfaces, the key molecular events describing how adhesive molecules act on plasma membrane to generate strength is still elusive and remains controversial. In addition to the classic cellular and molecular tools, quantitative measurement of functionality for cell adhesion in a better controlled condition of time and space is essential for revealing complex and dynamic molecular cascades. Thus, we have developed mechanobiological methods and protocols, in one way aiming at measuring mechanical parameters for uncovering cell adhesion property (e.g., dual micropipette assay, Chu et al, 2004, 2005,2006), and the other, testing  cell responses in function of applied forces for revealing mechanosensing and mechanotranscution mechanism (e.g., stretchable substrate, Thomas et al, 2012) at molecular and cellular level. Through inter-disciplinary research methodologies, we have recently identified a novel associating molecule, utrophin, an F-actin binding molecule, with p120 catenin of E-cadherin complexes and able to regulate the Cadherin-based cell adhesivity (Chu et al, manuscript in preparation). Furthermore, we have generated a list of Cadherin intereactome by immunoprecipitation and mass-spec.  The current research plan is to validate the critical leads able to regulate the cell-cell adhesion strength. Our joint efforts are not only dedicating to identify novel molecular mechanisms of controlling cell adhesion, but also aiming at delineating biophysical principles governing the construction and destruction of adhesion structures from basic adhesive units at adhesive interfaces.

SELECTED PUBLICATIONS in the past 5 years
  1. Huang RY, Wong MK, Tan TZ, Kuay KT, Ng AH, Chung VY, Chu YS, Matsumura N, Lai HC, Lee YF, Sim WJ, Chai C, Pietschmann E, Mori S, Low JJ, Choolani M, Thiery JP. An EMT spectrum defines an anoikis-resistant and spheroidogenic intermediate mesenchymal state that is sensitive to e-cadherin restoration by a src-kinase inhibitor, saracatinib (AZD0530). Cell Death Dis. 4:e915. 2013
  2. Voon DC, Wang H, Koo JK, Chai JH, Hor YT, Tan TZ, Chu YS, Mori S, Ito Y.EMT-induced stemness and tumorigenicity are fueled by the EGFR/Ras pathway, PLoS One. 28(8):e70427, 2013

  3. Lin L, Chu YS, Thiery JP, Lim CT, Rodriguez I. Microfluidic cell trap array for controlled positioning of single cells on adhesive micropatterns. Lab on a Chip 13:714-212013

  4. Thomas WA. Boscher C, Chu YS, Cuvelier D, Martinez-Rico C, Seddiki R, Heysch J,  Ladoux B, Thiery JP, Mege RM, Dufour S. α-catenin and vinculin cooperate to promote high E-cadherin-based adhesion strength.  J. Biol. Chem. 288:4957-69, 2013

  5.  Maiuri P, Terriac E, Paul-Gilloteaux P, Vignaud T, McNally K, Onuffer J, Thorn K, Nguyen PA, Georgoulia N, Soong D, Jayo A, Beil N, Beneke J, Lim JC, Sim CP, Chu YS; WCR participants, Jiménez-Dalmaroni A, Joanny JF, Thiery JP, Erfle H, Parsons M, Mitchison TJ, Lim WA, Lennon-Duménil AM, Piel M, Théry M. The first World Cell Race. Current Biology22(17):R673-5, 2012

  6. Voon DC, Wang H, Koo JK, Nguyen TA, Hor YT, Chu YS, Ito K, Fukamachi H, Chan SL, Thiery JP, Ito Y. Runx3 protects gastric epithelial cells against epithelial-mesenchymal transition-induced cellular plasticity and tumorigenicity. Stem Cells. 30(10):2088-99, 2012.

  7. Racine J, Cheradame H, Chu YS, Thiery JPRodriguez I. Cell adhesive micropatterns defined by poly-(ethylene oxide)-block-poly (4-vinylpyridine) diblock copolymer, Biotechnology and Bioengineering,108:983-9872011

  8. Racine J, Luong-Van E, Sadikin Y, Kang RKC, Chu YS, Racine V, Thiery JP, Birch WR.  A versatile gradient of biomolecules for regulating cell behaviour, Journal of Adhesion Science and Technology, 24: 975-992, 2010.

  9. Kiew JS, Sui X, Chu YS, Thiery JP, Rodriguez I. Fabrication of adhesive protein micropatterns in application of studying cell surface interactions, IFMBE Proceedings, 23:1980-1983, 2009.